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Flashcards; Learn; Test; Match; Q-Chat; 1- use the incoulum from the gram positive stock plate 2- transfer a loop of inoculum onto a clean microscope slide 3- add one drop of 3% H2O2 on to the bacteria slide and watch for bubbling. Total magnification is. Flashcards; Learn; Test; Match; Q-Chat; Created by. 117 terms. BIOLOGY 314. Skip to main Microbiology BIOS242 Final Lab Practical Exam Review. Study with Quizlet and memorize flashcards containing terms like Fixing kills the bacteria & allows it to stick to the slide; bacteria can be heat-fixed or chemically fixed with 95% methanol. Objective-nosepiece lenses (commonly 4x, 10x, 45x, and 100x) At what magnification do you use the oil immersion technique? - 100x. Exam (elaborations) - Microbiology lab Study with Quizlet and memorize flashcards containing terms like Which term describes a round-shaped bacterium?, Colonies are observed by looking at a plate. Objective lens × 10. 1 / 100. KOEHLER 1. Study guide. 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